A simple, rapid, accurate, precise and economical reverse phase high performance liquid chromatographic method is developed for simultaneous separation and quantification of two anti-hypertensive drugs, viz., chlorthalidone and nebivolol hydrochloride. The separation of both the drugs was achieved on Zorbax eclipse XDB C8 column (250 x 4.6 mm id, 5 µm particle size) column using a mobile phase of Potassium dihydrogen phosphate and triethylamine buffer solution (at pH 3): acetonitrile (65:35 v/v). The flow rate was 1 ml/min and detection was done at 280 nm. The retention time for chlorthalidone was 4.36 min and nebivolol hydrochloride was 9.68 min. Chlorthalidone and nebivolol hydrochloride showed a linear response in the concentration range of 31.25-187.5 µg/ml and 12.5-75 µg/ml respectively. The correlation co-efficients for Chlorthalidone and Nebivolol hydrochloride were 0.9998 and 0.9999 respectively. The percentage recoveries obtained for chlorthalidone and nebivolol hydrochloride ranges from 99.4% to 100.5% and 99.4% to 99.8% respectively. The results of analysis have been validated as per ICH guidelines. Validation results indicated that method shows satisfactory linearity, accuracy, precision and ruggedness. The extremely low flow rate, simple mobile phase composition makes this method cost effective, rapid and non-tedious and can also be successfully employed for simultaneous estimation of both drugs in commercial products.
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